Ownership Submission
FORM 4
Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).
UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940
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(Print or Type Responses)
1. Name and Address of Reporting Person *
Esfandiari Javan
   2. Issuer Name and Ticker or Trading Symbol
CHEMBIO DIAGNOSTICS, INC. [CEMI] 		5. Relationship of Reporting Person(s) to Issuer
(Check all applicable)
_____ Director _____ 10% Owner
__X__ Officer (give title below) _____ Other (specify below)
Senior Vice President
(Last)
(First)
(Middle)
3661 HORSEBLOCK ROAD		 3. Date of Earliest Transaction (Month/Day/Year)
05/07/2009
(Street)

MEDFORD, NY 11763		 4. If Amendment, Date Original Filed(Month/Day/Year)
 6. Individual or Joint/Group Filing(Check Applicable Line)
_X_ Form filed by One Reporting Person
___ Form filed by More than One Reporting Person
(City)
(State)
(Zip)
Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1.Title of Security
(Instr. 3)		 2. Transaction Date (Month/Day/Year) 2A. Deemed Execution Date, if any (Month/Day/Year) 3. Transaction Code
(Instr. 8)		 4. Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4 and 5)		 5. Amount of Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 3 and 4)		 6. Ownership Form: Direct (D) or Indirect (I)
(Instr. 4)		 7. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V Amount (A) or (D) Price

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
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Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned
(e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security
(Instr. 3)		 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (Month/Day/Year) 3A. Deemed Execution Date, if any (Month/Day/Year) 4. Transaction Code
(Instr. 8)		 5. Number of Derivative Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4, and 5)		 6. Date Exercisable and Expiration Date
(Month/Day/Year)		 7. Title and Amount of Underlying Securities
(Instr. 3 and 4)		 8. Price of Derivative Security
(Instr. 5)		 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 4)		 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 4)		 11. Nature of Indirect Beneficial Ownership
(Instr. 4)
Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares
Options (right to buy) $ 0.48 05/07/2009   D     50,000   (2) 05/17/2010 Common Stock 50,000 (1) 0 D  
Options (right to buy) $ 0.48 05/07/2009   D     37,500   (3) 03/24/2011 Common Stock 37,500 (1) 0 D  
Options (right to buy) $ 0.48 05/07/2009   D     35,000   (4) 05/04/2011 Common Stock 35,000 (1) 0 D  
Options (right to buy) $ 0.48 05/07/2009   D     75,000   (5) 05/28/2011 Common Stock 75,000 (1) 0 D  
Options (right to buy) $ 0.48 05/07/2009   D     300,000   (6) 04/23/2012 Common Stock 300,000 (1) 0 D  
Options (right to buy) $ 0.13 05/07/2009   A   50,000     (2) 05/17/2010 Common Stock 50,000 $ 0.13 50,000 D  
Options (right to buy) $ 0.13 05/07/2009   A   37,500     (3) 03/24/2011 Common Stock 37,500 $ 0.13 37,500 D  
Options (right to buy) $ 0.13 05/07/2009   A   35,000     (4) 05/04/2011(4) Common Stock 35,000 $ 0.13 37,500 D  
Options (right to buy) $ 0.13 05/07/2009   A   75,000     (5) 05/28/2011 Common Stock 75,000 $ 0.13 75,000 D  
Options (right to buy) $ 0.13 05/07/2009   A   300,000     (6) 04/23/2012 Common Stock 300,000 $ 0.13 300,000 D  
Options (right to buy) $ 0.13 05/07/2009   A   300,000     (7) 05/07/2014 Common Stock 300,000 $ 0.13 300,000 D  

Reporting Owners

Reporting Owner Name / Address Relationships
 Director  10% Owner  Officer  Other
Esfandiari Javan
3661 HORSEBLOCK ROAD
MEDFORD, NY 11763		       Senior Vice President  

Signatures

 /s/ Javan Esfandiari   11/03/2009
**Signature of Reporting Person Date

Explanation of Responses:

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1) The reporting person agreed to cancellation of an option granted to him in exchange for a new option having a lower price.
(2) 25,000 options vested on each of 04/17/06 and 1/1/07
(3) 18,750 options vested on each of 3/24/06 and 01/01/07
(4) 30,000 options vested on 05/5/04 and 5,000 options vested on 4/17/06
(5) 25,000 options vested on each of 04/17/06, 05/28/06 and 05/28/07
(6) 100,000 options vested on each of 04/23/07, 03/15/08, 03/05/09
(7) 100,000 options vest on each of 05/07/10, 05/07/11, and 05/07/12

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